Abstract:Abstract: Epithelial-mesenchymal transition (EMT) is an important biological process for the migration and invasion of epithelial-derived malignant cells. In order to study the molecular mechanism of Krüppel-like factor 5 (KLF5) in regulating the EMT process in cervical cancer cells, the effect of KLF5 gene on proliferation, invasion and metastasis of cervical cancer cells was detected by MTT, cell scratch wound and Transwell methods after overexpression of KLF5 gene in HeLa cells. It was found that KLF5 significantly inhibited the invasion and migration of HeLa cells. Then, Western-blot and real-time quantitative PCR were used to detect the expression of EMT-related genes in HeLa cells. It proved that the expression of E-cadherin was increased while N-cadherin and MMP9 expressions were decreased, and that the mRNA expression of upstream regulatory factors such as SNAI1, SLUG, ZEB1/2 and TWIST1 of E-cadherin gene was also decreased. Moreover, the expression changes of EMT-related genes were examined again by silencing of the KLF5 gene with siRNA in SiHa cells. Subsequently, the constructed SNAI1 promoter truncations of different lengths were used to detect the effect of KLF5 on the activity of SNAI1 promoter by luciferase reporter gene. The results showed that KLF5 inhibited the activity of SNAI1 promoter region, and that the EMT process of cells was promoted after overexpression of SNAI1 gene in HeLa cells. These results indicate that KLF5 can down-regulate the EMT process of cervical cancer cells by decreasing the expression of SNAI1 gene, thereby inhibiting the migration and invasion of cervical cancer cells.
引用本文:
杨文博, 李芊芊, 王 键, 曲鑫建. 转录因子KLF5调控宫颈癌细胞上皮-间充质转化的分子机制[J]. 生命科学研究, 2019, 23(3): 183-191.
YANG Wen-bo, LI Qian-qian, WANG Jian, QU Xin-jian. Molecular Mechanism of KLF5 in Regulation of Epithelial-Mesenchymal Transition in Cervical Cancer Cells. Life Science Research, 2019, 23(3): 183-191.