Development of Polymorphic Microsatellite Markers for Sinoswertia tetraptera (Gentianaceae)
1. College of Ecological Environment and Resources, Qinghai Minzu University, Xining 810007, Qinghai, China; 2. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China; 3. College of Pharmacy, Qinghai Minzu University, Xining 810007, Qinghai, China; 4. Qinghai Provincial Key Laboratory of High-value Utilization of Characteristic Economic Plants, Xining 810007, Qinghai, China
Abstract：Sinoswertia tetraptera (Maximowicz) T. N. Ho, S. W. Liu & J. Q. Liu is an annual herb endemic to the Qinghai-Tibet Plateau. Here, microsatellite (simple sequence repeat, SSR) markers for S. tetraptera were developed in order to better utilize and protect this essential medicinal plant resource. Primers for 14 polymorphic and 2 monomorphic microsatellite loci were obtained based on restriction site-associated DNA se-quencing (RAD-seq). The 14 polymorphic loci were further investigated in 57 individuals from three natural populations of S. tetraptera. The number of alleles observed for these 14 loci ranged from 4 to 7 (mean: 5.071). The expected heterozygosity ranged from 0.003 to 0.312 (mean: 0.194), while the observed heterozy-gosity ranged from 0 to 0.281 (mean: 0.047). Three primers showed transferability to Halenia elliptica, a mem-ber of the subtribe Swertiinae (Gentianaceae). These polymorphic markers will be useful in future research on the population structure and genetic diversity of S. tetraptera.
王久利, 张 鑫, 王旭乾, 王丽蓉. 异型花多态性微卫星标记的开发（英文）[J]. 生命科学研究, 2023, 27(1): 80-85.
WANG Jiuli, ZHANG Xin, WANG Xuqian, WANG Lirong. Development of Polymorphic Microsatellite Markers for Sinoswertia tetraptera (Gentianaceae). Life Science Research, 2023, 27(1): 80-85.
