Abstract:Abstract: Several recent studies have revealed that the symbiotic microbes may be lost during domestication and long years of continuous cultivation, leaving their hosts more vulnerable to stress and diseases, and that acquisition of microbes from the corresponding wild plants could restore host resistance to biotic and abiotic stresses. Burkholderia are found in a wide range of terrestrial and aquatic niches, they could produce a plethora of natural product with diverse structure and activity. Some Burkholderia strains are capable of fixing nitrogen, while some exhibit activity against arthropod pests. Herein, the rhizosphere soil sample was collected from wild Gramineae plants for targeted isolation of antagonistic bacteria belonging to Burkholderia. Among the isolated Burkholderia strains, the Burkholderia sp. Z1 showed a broad spectrum of antagonistic activity against phytopathogenic fungi. According to the genetic analysis and the phenotypic characteristics, Z1 had a high possibility of being a new member of the Burkholderia cepacia complex genomovar Ⅸ, the Burkholde-ria pyrrocinia. High resolution mass spectrum analysis and the hemolytic activity results on sheep blood agar indicated its antifungal activity could be attributed to the production of occidiofungins and burkholdines, in-cluding several new derivatives. To our best knowledge, though these two kinds of cyclic lipopeptides were similar in structure, this is the first report showing one strain could produce both of them. Its genome proved to harbor a gene cluster highly similar to the occidiofungin biosynthetic gene cluster.
引用本文:
唐 滢, 雷 平, 张翠央, 毕世宇, 郭照辉, 刘清术. 抑真菌伯克霍尔德氏菌Z1的分离鉴定及其抑菌物质分析[J]. 生命科学研究, 2020, 24(6): 484-493.
TANG Ying, LEI Ping, ZHANG Cui-yang, BI Shi-yu, GUO Zhao-hui, LIU Qing-shu. Isolation and Identification of Burkholderia sp. Z1 and Analysis of Its Antifungal Components . Life Science Research, 2020, 24(6): 484-493.